解剖学报

›› 2009, Vol. 40 ›› Issue (6): 928-932.doi: 10.3969/j.issn.0529-1356.2009.06.016

• 论著 • 上一篇    下一篇

Grp78表达对人肝细胞癌细胞外信号调节激酶1/2活性和表达的影响

苏荣健1*; 李贞1,2; 程留芳2 ;李宏丹1 ;魏嘉1 ;包翠芬1   

  1. 1.辽宁医学院科学实验中心 细胞和分子生物学与新药开发重点实验室，辽宁锦州 121001;2.解放军总医院消化科，北京 100583
  • 收稿日期:2008-12-16 修回日期:2009-02-25 出版日期:2009-12-06
  • 通讯作者: SU Rong-jian

Effect of Grp78 on the activation and expression of ERK 1/2 in human hepatocellular carcinoma tissues

  1. 1.Central Laboratory of Liaoning Medical College and Key Laboratory of Cell and Molecular Biology and Drug Development of Education Supervisory Office of Liaoning Province, Jinzhou 121001,China；2.Department of Gastroenterology, General Hospital of Chinese Liberation Army, Beijing 100583,China
  • Received:2008-12-16 Revised:2009-02-25 Online:2009-12-06

关键词: 肝细胞癌, Grp78, 细胞外信号调节激酶1/2, 小RNA干扰, SMMC-7721, 免疫组织化学, 免疫印迹法, 人

Abstract: Objective We examined the Grp78, ERK1/2 and phospho-ERK1/2 expressions in hepatocellular carcinoma(HCC) tissue samples EM>in vitro/EM>, we interfered the expression of Grp78 in SMMC-7721 cells to explore whether Grp78 is involved in ERK1/2 signal pathway. Methods The Grp78, ERK1/2 and phospho-ERK1/2 expressions were detected by immunohistochemistry and confirmed by Western blotting in 47 HCC tissue samples. The Grp78 expression in SMMC-7721 cells was interfered by plasmid transfection and siRNA, ERK1/2 phosphorylation and expression were determined by Western blotting. Results The Grp78 expression was significantly correlated with ERK1/2 and phospho-ERK1/2 in HCC tissue samples. Overexpression of Grp78 promoted ERK1/2 phosphorylation in SMMC-7721 cells and the increased ERK1/2 phosphorylation was inhibited by Grp78 knockdown. Conclusion Grp78 is involved in the regulation of ERK1/2 signal pathway and might be a potential target for the comprehensive therapy of HCC.

Key words: Hepatocellular carcinoma, Grp78, ERK1/2, siRNA, SMMC-7721, Immunohistochemistry, Western blotting, Human

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